Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by...

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Autores principales: Trumpp, Michael, Oliveras, Anna, Gonschior, Hannes, Ast, Julia, Hodson, David J., Knaus, Petra, Lehmann, Martin, Birol, Melissa, Broichhagen, Johannes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745879/
https://www.ncbi.nlm.nih.gov/pubmed/36427021
http://dx.doi.org/10.1039/d2cc04823j
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author Trumpp, Michael
Oliveras, Anna
Gonschior, Hannes
Ast, Julia
Hodson, David J.
Knaus, Petra
Lehmann, Martin
Birol, Melissa
Broichhagen, Johannes
author_facet Trumpp, Michael
Oliveras, Anna
Gonschior, Hannes
Ast, Julia
Hodson, David J.
Knaus, Petra
Lehmann, Martin
Birol, Melissa
Broichhagen, Johannes
author_sort Trumpp, Michael
collection PubMed
description Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.
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spelling pubmed-97458792022-12-20 Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy Trumpp, Michael Oliveras, Anna Gonschior, Hannes Ast, Julia Hodson, David J. Knaus, Petra Lehmann, Martin Birol, Melissa Broichhagen, Johannes Chem Commun (Camb) Chemistry Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy. The Royal Society of Chemistry 2022-11-21 /pmc/articles/PMC9745879/ /pubmed/36427021 http://dx.doi.org/10.1039/d2cc04823j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Trumpp, Michael
Oliveras, Anna
Gonschior, Hannes
Ast, Julia
Hodson, David J.
Knaus, Petra
Lehmann, Martin
Birol, Melissa
Broichhagen, Johannes
Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
title Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
title_full Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
title_fullStr Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
title_full_unstemmed Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
title_short Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
title_sort enzyme self-label-bound atto700 in single-molecule and super-resolution microscopy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745879/
https://www.ncbi.nlm.nih.gov/pubmed/36427021
http://dx.doi.org/10.1039/d2cc04823j
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