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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy
Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by...
Autores principales: | Trumpp, Michael, Oliveras, Anna, Gonschior, Hannes, Ast, Julia, Hodson, David J., Knaus, Petra, Lehmann, Martin, Birol, Melissa, Broichhagen, Johannes |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9745879/ https://www.ncbi.nlm.nih.gov/pubmed/36427021 http://dx.doi.org/10.1039/d2cc04823j |
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