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Flow cytometric reporter assays provide robust functional analysis of signaling complexes

Conventional assays to probe signaling protein interactions and function involve measurement of luciferase reporter expression within the bulk cell population, with lack of control over target-protein expression level. To address this issue, we have developed a rapid and robust flow cytometric assay...

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Detalles Bibliográficos
Autores principales: Muusse, Timothy W., Lee, Morris Y.L., Kim, Hyoyoung, Parat, Marie-Odile, Nanson, Jeffrey D., Kobe, Bostjan, Vajjhala, Parimala R., Stacey, Katryn J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9747584/
https://www.ncbi.nlm.nih.gov/pubmed/36334634
http://dx.doi.org/10.1016/j.jbc.2022.102666