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An advanced and efficient asymmetric PCR method for microarray applications

The sensitivity of a PCR based biochip assay relies on the efficiency of PCR amplicons in binding to the microarray spots. The essential factor determining the sensitivity is the amount of single stranded (ss) amplicons available for biochip hybridization. Asymmetric PCR can generate ss-amplicons de...

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Detalles Bibliográficos
Autores principales: Reddy Banda, Suresh, Klapproth, Holger, Smit, Nicolaas, Bednar, Sonja, Brandstetter, Thomas, Rühe, Jürgen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748121/
https://www.ncbi.nlm.nih.gov/pubmed/36532575
http://dx.doi.org/10.3389/fbioe.2022.1045154