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An advanced and efficient asymmetric PCR method for microarray applications
The sensitivity of a PCR based biochip assay relies on the efficiency of PCR amplicons in binding to the microarray spots. The essential factor determining the sensitivity is the amount of single stranded (ss) amplicons available for biochip hybridization. Asymmetric PCR can generate ss-amplicons de...
Autores principales: | Reddy Banda, Suresh, Klapproth, Holger, Smit, Nicolaas, Bednar, Sonja, Brandstetter, Thomas, Rühe, Jürgen |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9748121/ https://www.ncbi.nlm.nih.gov/pubmed/36532575 http://dx.doi.org/10.3389/fbioe.2022.1045154 |
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