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High-throughput total RNA sequencing in single cells using VASA-seq

Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes the detection of many long non-coding, short non-coding and non-polyadenylated protein-coding transcripts and h...

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Detalles Bibliográficos
Autores principales: Salmen, Fredrik, De Jonghe, Joachim, Kaminski, Tomasz S., Alemany, Anna, Parada, Guillermo E., Verity-Legg, Joe, Yanagida, Ayaka, Kohler, Timo N., Battich, Nicholas, van den Brekel, Floris, Ellermann, Anna L., Arias, Alfonso Martinez, Nichols, Jennifer, Hemberg, Martin, Hollfelder, Florian, van Oudenaarden, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9750877/
https://www.ncbi.nlm.nih.gov/pubmed/35760914
http://dx.doi.org/10.1038/s41587-022-01361-8