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High-throughput total RNA sequencing in single cells using VASA-seq
Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes the detection of many long non-coding, short non-coding and non-polyadenylated protein-coding transcripts and h...
Autores principales: | Salmen, Fredrik, De Jonghe, Joachim, Kaminski, Tomasz S., Alemany, Anna, Parada, Guillermo E., Verity-Legg, Joe, Yanagida, Ayaka, Kohler, Timo N., Battich, Nicholas, van den Brekel, Floris, Ellermann, Anna L., Arias, Alfonso Martinez, Nichols, Jennifer, Hemberg, Martin, Hollfelder, Florian, van Oudenaarden, Alexander |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9750877/ https://www.ncbi.nlm.nih.gov/pubmed/35760914 http://dx.doi.org/10.1038/s41587-022-01361-8 |
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