Protocol for investigating the biogenesis of SARS-CoV-2 S pseudoviruses in HEK293T cells transduced to express the virus-specific intrabodies

The protocol is designed to investigate the influence of an anti-cleavage site intrabody in modulating the output of LV(CoV-2 S), a lentivirus-based pseudovirus expressing CoV-2 S protein using HEK293T cells. We clone the single-domain antibody sequence into a lentiviral vector (pLenti-GFP) for intr...

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Detalles Bibliográficos
Autores principales: Dahiya, Surbhi, Singh, Sudhakar, Sehrawat, Sharvan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9750881/
https://www.ncbi.nlm.nih.gov/pubmed/36587286
http://dx.doi.org/10.1016/j.xpro.2022.101977

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9750881/
https://www.ncbi.nlm.nih.gov/pubmed/36587286
http://dx.doi.org/10.1016/j.xpro.2022.101977

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