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Effects of Polyphenols and Glucosinolates in Broccoli Extract on Human Gut Microorganisms Based on Simulation In Vitro
[Image: see text] Broccoli extract mainly contains polyphenols and glucosinolates (GSLs). GSLs can be hydrolyzed by gut microorganisms into isothiocyanates (ITCs) and other active substances. These substances have anticancer, anti-inflammatory, antimicrobial, and atherosclerosis-reducing functions....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753209/ https://www.ncbi.nlm.nih.gov/pubmed/36530270 http://dx.doi.org/10.1021/acsomega.2c05523 |
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author | Zhang, Yao Jiang, Chunmin Huang, Sisi Sun, Juan Song, Xinjie Nishanbaev, Sabir Z. Benito, María Jose Wu, Yuanfeng |
author_facet | Zhang, Yao Jiang, Chunmin Huang, Sisi Sun, Juan Song, Xinjie Nishanbaev, Sabir Z. Benito, María Jose Wu, Yuanfeng |
author_sort | Zhang, Yao |
collection | PubMed |
description | [Image: see text] Broccoli extract mainly contains polyphenols and glucosinolates (GSLs). GSLs can be hydrolyzed by gut microorganisms into isothiocyanates (ITCs) and other active substances. These substances have anticancer, anti-inflammatory, antimicrobial, and atherosclerosis-reducing functions. In this study, a high concentration (2000 μmol/L GSLs and 24 μmol/L polyphenols) and a low concentration (83 μmol/L GSLs and 1 μmol/L polyphenols) of broccoli extract were prepared. Gut microorganisms from fresh human feces were cultured to simulate the gut environment in vitro. The GSL content decreased and the types and content of ITCs increased with broccoli extract hydrolysis through cyclic condensation and gas chromatography–mass spectrometry (GC-MS) analyses. Broccoli extract significantly increased probiotics and inhibited harmful bacteria through 16S rDNA sequencing. Based on phylum level analysis, Firmicutes and Lachnospiraceae increased significantly (P < 0.05). At the genus level, both high- and low-concentration groups significantly inhibited Escherichia and increased Bilophila and Alistipes (P < 0.05). The high-concentration group significantly increased Bifidobacterium (P < 0.05). The broccoli extract improved the richness of gut microorganisms and regulated their structure. The GSL hydrolysis was significantly correlated with Bilophila, Lachnospiraceae, Alistipes, Bifidobacterium, Escherichia, and Streptococcus (P < 0.05). These study findings provide a theoretical foundation for further exploring a probiotic mechanism of broccoli extract in the intestine. |
format | Online Article Text |
id | pubmed-9753209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-97532092022-12-16 Effects of Polyphenols and Glucosinolates in Broccoli Extract on Human Gut Microorganisms Based on Simulation In Vitro Zhang, Yao Jiang, Chunmin Huang, Sisi Sun, Juan Song, Xinjie Nishanbaev, Sabir Z. Benito, María Jose Wu, Yuanfeng ACS Omega [Image: see text] Broccoli extract mainly contains polyphenols and glucosinolates (GSLs). GSLs can be hydrolyzed by gut microorganisms into isothiocyanates (ITCs) and other active substances. These substances have anticancer, anti-inflammatory, antimicrobial, and atherosclerosis-reducing functions. In this study, a high concentration (2000 μmol/L GSLs and 24 μmol/L polyphenols) and a low concentration (83 μmol/L GSLs and 1 μmol/L polyphenols) of broccoli extract were prepared. Gut microorganisms from fresh human feces were cultured to simulate the gut environment in vitro. The GSL content decreased and the types and content of ITCs increased with broccoli extract hydrolysis through cyclic condensation and gas chromatography–mass spectrometry (GC-MS) analyses. Broccoli extract significantly increased probiotics and inhibited harmful bacteria through 16S rDNA sequencing. Based on phylum level analysis, Firmicutes and Lachnospiraceae increased significantly (P < 0.05). At the genus level, both high- and low-concentration groups significantly inhibited Escherichia and increased Bilophila and Alistipes (P < 0.05). The high-concentration group significantly increased Bifidobacterium (P < 0.05). The broccoli extract improved the richness of gut microorganisms and regulated their structure. The GSL hydrolysis was significantly correlated with Bilophila, Lachnospiraceae, Alistipes, Bifidobacterium, Escherichia, and Streptococcus (P < 0.05). These study findings provide a theoretical foundation for further exploring a probiotic mechanism of broccoli extract in the intestine. American Chemical Society 2022-12-02 /pmc/articles/PMC9753209/ /pubmed/36530270 http://dx.doi.org/10.1021/acsomega.2c05523 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Zhang, Yao Jiang, Chunmin Huang, Sisi Sun, Juan Song, Xinjie Nishanbaev, Sabir Z. Benito, María Jose Wu, Yuanfeng Effects of Polyphenols and Glucosinolates in Broccoli Extract on Human Gut Microorganisms Based on Simulation In Vitro |
title | Effects of Polyphenols
and Glucosinolates in Broccoli
Extract on Human Gut Microorganisms Based on Simulation In Vitro |
title_full | Effects of Polyphenols
and Glucosinolates in Broccoli
Extract on Human Gut Microorganisms Based on Simulation In Vitro |
title_fullStr | Effects of Polyphenols
and Glucosinolates in Broccoli
Extract on Human Gut Microorganisms Based on Simulation In Vitro |
title_full_unstemmed | Effects of Polyphenols
and Glucosinolates in Broccoli
Extract on Human Gut Microorganisms Based on Simulation In Vitro |
title_short | Effects of Polyphenols
and Glucosinolates in Broccoli
Extract on Human Gut Microorganisms Based on Simulation In Vitro |
title_sort | effects of polyphenols
and glucosinolates in broccoli
extract on human gut microorganisms based on simulation in vitro |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753209/ https://www.ncbi.nlm.nih.gov/pubmed/36530270 http://dx.doi.org/10.1021/acsomega.2c05523 |
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