Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains

BACKGROUND: Owing to CRISPR-Cas9 and derivative technologies, genetic studies on microorganisms have dramatically increased. However, the CRISPR-Cas9 system is still difficult to utilize in many wild-type Bacillus strains owing to Cas9 toxicity. Moreover, less toxic systems, such as cytosine base ed...

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Detalles Bibliográficos
Autores principales: Kim, Man Su, Jeong, Da-Eun, Choi, Soo-Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753358/
https://www.ncbi.nlm.nih.gov/pubmed/36517844
http://dx.doi.org/10.1186/s12934-022-01989-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753358/
https://www.ncbi.nlm.nih.gov/pubmed/36517844
http://dx.doi.org/10.1186/s12934-022-01989-w

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