Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System

A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible cas9 gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology fo...

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Detalles Bibliográficos
Autores principales: Brehm, Joshua N., Sorg, Joseph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753633/
https://www.ncbi.nlm.nih.gov/pubmed/36342279
http://dx.doi.org/10.1128/mra.00833-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753633/
https://www.ncbi.nlm.nih.gov/pubmed/36342279
http://dx.doi.org/10.1128/mra.00833-22

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