Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System

A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible cas9 gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology fo...

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Autores principales: Brehm, Joshua N., Sorg, Joseph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753633/
https://www.ncbi.nlm.nih.gov/pubmed/36342279
http://dx.doi.org/10.1128/mra.00833-22
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author Brehm, Joshua N.
Sorg, Joseph A.
author_facet Brehm, Joshua N.
Sorg, Joseph A.
author_sort Brehm, Joshua N.
collection PubMed
description A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible cas9 gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology for repair of the introduced double-stranded DNA (dsDNA) breaks, both of which are replaceable via restriction digest.
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spelling pubmed-97536332022-12-16 Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System Brehm, Joshua N. Sorg, Joseph A. Microbiol Resour Announc Other Genetic Resources A two-plasmid mutagenesis system for Clostridioides difficile is described that improves ease of use and efficiency in creating site-directed mutations. pJB06 contains a xylose-inducible cas9 gene, while the second plasmid (pJB07) encodes the corresponding guide RNA (gRNA) and regions of homology for repair of the introduced double-stranded DNA (dsDNA) breaks, both of which are replaceable via restriction digest. American Society for Microbiology 2022-11-07 /pmc/articles/PMC9753633/ /pubmed/36342279 http://dx.doi.org/10.1128/mra.00833-22 Text en Copyright © 2022 Brehm and Sorg. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Other Genetic Resources
Brehm, Joshua N.
Sorg, Joseph A.
Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System
title Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System
title_full Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System
title_fullStr Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System
title_full_unstemmed Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System
title_short Plasmid Sequence and Availability for an Improved Clostridioides difficile CRISPR-Cas9 Mutagenesis System
title_sort plasmid sequence and availability for an improved clostridioides difficile crispr-cas9 mutagenesis system
topic Other Genetic Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9753633/
https://www.ncbi.nlm.nih.gov/pubmed/36342279
http://dx.doi.org/10.1128/mra.00833-22
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