Bidirectional promoter activity from expression cassettes can drive off-target repression of neighboring gene translation

Targeted selection-based genome-editing approaches have enabled many fundamental discoveries and are used routinely with high precision. We found, however, that replacement of DBP1 with a common selection cassette in budding yeast led to reduced expression and function for the adjacent gene, MRP51,...

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Detalles Bibliográficos
Autores principales: Powers, Emily Nicole, Chan, Charlene, Doron-Mandel, Ella, Llacsahuanga Allcca, Lidia, Kim Kim, Jenny, Jovanovic, Marko, Brar, Gloria Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Chromosomes and Gene Expression
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754628/
https://www.ncbi.nlm.nih.gov/pubmed/36503721
http://dx.doi.org/10.7554/eLife.81086

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754628/
https://www.ncbi.nlm.nih.gov/pubmed/36503721
http://dx.doi.org/10.7554/eLife.81086

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