Identification of secreted proteins by comparison of protein abundance in conditioned media and cell lysates

Analysis of the full spectrum of secreted proteins in cell culture is complicated by leakage of intracellular proteins from damaged cells. To address this issue, we compared the abundance of individual proteins between the cell lysate and the conditioned medium, reasoning that secreted proteins shou...

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Detalles Bibliográficos
Autores principales: Abbineni, Prabhodh S., Tang, Vi T., da Veiga Leprevost, Felipe, Basrur, Venkatesha, Xiang, Jie, Nesvizhskii, Alexey I., Ginsburg, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756135/
https://www.ncbi.nlm.nih.gov/pubmed/35973625
http://dx.doi.org/10.1016/j.ab.2022.114846

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756135/
https://www.ncbi.nlm.nih.gov/pubmed/35973625
http://dx.doi.org/10.1016/j.ab.2022.114846

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