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Identification of secreted proteins by comparison of protein abundance in conditioned media and cell lysates
Analysis of the full spectrum of secreted proteins in cell culture is complicated by leakage of intracellular proteins from damaged cells. To address this issue, we compared the abundance of individual proteins between the cell lysate and the conditioned medium, reasoning that secreted proteins shou...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9756135/ https://www.ncbi.nlm.nih.gov/pubmed/35973625 http://dx.doi.org/10.1016/j.ab.2022.114846 |