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The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis

α-Catenin couples the cadherin–catenin complex to the actin cytoskeleton. The mechanosensitive α-Catenin M region undergoes conformational changes upon application of force to recruit interaction partners. Here, we took advantage of the tension landscape in the Drosophila embryo to define three diff...

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Autores principales: Sheppard, Luka, Green, David G., Lerchbaumer, Gerald, Rothenberg, Katheryn E., Fernandez-Gonzalez, Rodrigo, Tepass, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9757846/
https://www.ncbi.nlm.nih.gov/pubmed/36520419
http://dx.doi.org/10.1083/jcb.202108091
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author Sheppard, Luka
Green, David G.
Lerchbaumer, Gerald
Rothenberg, Katheryn E.
Fernandez-Gonzalez, Rodrigo
Tepass, Ulrich
author_facet Sheppard, Luka
Green, David G.
Lerchbaumer, Gerald
Rothenberg, Katheryn E.
Fernandez-Gonzalez, Rodrigo
Tepass, Ulrich
author_sort Sheppard, Luka
collection PubMed
description α-Catenin couples the cadherin–catenin complex to the actin cytoskeleton. The mechanosensitive α-Catenin M region undergoes conformational changes upon application of force to recruit interaction partners. Here, we took advantage of the tension landscape in the Drosophila embryo to define three different states of α-Catenin mechanosensing in support of cell adhesion. Low-, medium-, and high-tension contacts showed a corresponding recruitment of Vinculin and Ajuba, which was dependent on the α-Catenin M region. In contrast, the Afadin homolog Canoe acts in parallel to α-Catenin at bicellular low- and medium-tension junctions but requires an interaction with α-Catenin for its tension-sensitive enrichment at high-tension tricellular junctions. Individual M region domains make complex contributions to cell adhesion through their impact on interaction partner recruitment, and redundancies with the function of Canoe. Our data argue that α-Catenin and its interaction partners are part of a cooperative and partially redundant mechanoresponsive network that supports AJs remodeling during morphogenesis.
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spelling pubmed-97578462023-06-15 The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis Sheppard, Luka Green, David G. Lerchbaumer, Gerald Rothenberg, Katheryn E. Fernandez-Gonzalez, Rodrigo Tepass, Ulrich J Cell Biol Article α-Catenin couples the cadherin–catenin complex to the actin cytoskeleton. The mechanosensitive α-Catenin M region undergoes conformational changes upon application of force to recruit interaction partners. Here, we took advantage of the tension landscape in the Drosophila embryo to define three different states of α-Catenin mechanosensing in support of cell adhesion. Low-, medium-, and high-tension contacts showed a corresponding recruitment of Vinculin and Ajuba, which was dependent on the α-Catenin M region. In contrast, the Afadin homolog Canoe acts in parallel to α-Catenin at bicellular low- and medium-tension junctions but requires an interaction with α-Catenin for its tension-sensitive enrichment at high-tension tricellular junctions. Individual M region domains make complex contributions to cell adhesion through their impact on interaction partner recruitment, and redundancies with the function of Canoe. Our data argue that α-Catenin and its interaction partners are part of a cooperative and partially redundant mechanoresponsive network that supports AJs remodeling during morphogenesis. Rockefeller University Press 2022-12-15 /pmc/articles/PMC9757846/ /pubmed/36520419 http://dx.doi.org/10.1083/jcb.202108091 Text en © 2022 Sheppard et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Sheppard, Luka
Green, David G.
Lerchbaumer, Gerald
Rothenberg, Katheryn E.
Fernandez-Gonzalez, Rodrigo
Tepass, Ulrich
The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
title The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
title_full The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
title_fullStr The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
title_full_unstemmed The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
title_short The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis
title_sort α-catenin mechanosensing m region is required for cell adhesion during tissue morphogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9757846/
https://www.ncbi.nlm.nih.gov/pubmed/36520419
http://dx.doi.org/10.1083/jcb.202108091
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