A transcription-based approach to purify R-loop-containing plasmid DNA templates in vitro

To study the direct effects of R-loops on DNA replication and other DNA-templated processes in vitro, R-loop-containing DNA templates need to be prepared efficiently and to near homogeneity. Here, we describe a simple transcription-based approach to form R-loops on plasmid DNA templates in vitro. We...

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Detalles Bibliográficos
Autores principales: Kumar, Charanya, Remus, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758483/
https://www.ncbi.nlm.nih.gov/pubmed/36520635
http://dx.doi.org/10.1016/j.xpro.2022.101937

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758483/
https://www.ncbi.nlm.nih.gov/pubmed/36520635
http://dx.doi.org/10.1016/j.xpro.2022.101937

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