Cargando…
A transcription-based approach to purify R-loop-containing plasmid DNA templates in vitro
To study the direct effects of R-loops on DNA replication and other DNA-templated processes in vitro, R-loop-containing DNA templates need to be prepared efficiently and to near homogeneity. Here, we describe a simple transcription-based approach to form R-loops on plasmid DNA templates in vitro. We...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758483/ https://www.ncbi.nlm.nih.gov/pubmed/36520635 http://dx.doi.org/10.1016/j.xpro.2022.101937 |