Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE...

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Detalles Bibliográficos
Autores principales: Ge, Yong, Zadeh, Mojgan, Mohamadzadeh, Mansour
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758486/
https://www.ncbi.nlm.nih.gov/pubmed/36520632
http://dx.doi.org/10.1016/j.xpro.2022.101936

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758486/
https://www.ncbi.nlm.nih.gov/pubmed/36520632
http://dx.doi.org/10.1016/j.xpro.2022.101936

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