Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling

Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE...

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Autores principales: Ge, Yong, Zadeh, Mojgan, Mohamadzadeh, Mansour
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758486/
https://www.ncbi.nlm.nih.gov/pubmed/36520632
http://dx.doi.org/10.1016/j.xpro.2022.101936
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author Ge, Yong
Zadeh, Mojgan
Mohamadzadeh, Mansour
author_facet Ge, Yong
Zadeh, Mojgan
Mohamadzadeh, Mansour
author_sort Ge, Yong
collection PubMed
description Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).(1)
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spelling pubmed-97584862022-12-18 Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling Ge, Yong Zadeh, Mojgan Mohamadzadeh, Mansour STAR Protoc Protocol Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE Express, which can subsequently be employed for multi-omic analyses, including single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Ge et al. (2022).(1) Elsevier 2022-12-13 /pmc/articles/PMC9758486/ /pubmed/36520632 http://dx.doi.org/10.1016/j.xpro.2022.101936 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ge, Yong
Zadeh, Mojgan
Mohamadzadeh, Mansour
Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
title Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
title_full Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
title_fullStr Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
title_full_unstemmed Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
title_short Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
title_sort dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758486/
https://www.ncbi.nlm.nih.gov/pubmed/36520632
http://dx.doi.org/10.1016/j.xpro.2022.101936
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