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Dissociation and flow cytometric isolation of murine intestinal epithelial cells for multi-omic profiling
Intestinal epithelium is composed of several cell types, which can be dissociated but difficult to maintain high cell viability due to anoikis. Herein, we describe a step-by-step protocol for the isolation of highly viable intestinal epithelial cells using ethylenediaminetetraacetate acid and TrypLE...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758486/ https://www.ncbi.nlm.nih.gov/pubmed/36520632 http://dx.doi.org/10.1016/j.xpro.2022.101936 |