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Protocol to purify and detect ubiquitinated phospholipids in budding yeast and human cell lines
Ubiquitin is covalently conjugated to phospholipids as well as proteins; however, ubiquitinated phospholipids are less abundant than free ubiquitin and ubiquitinated proteins. Here, we describe protocols to purify ubiquitinated phospholipids in budding yeast and human cells based on their hydrophobi...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758488/ https://www.ncbi.nlm.nih.gov/pubmed/36520633 http://dx.doi.org/10.1016/j.xpro.2022.101935 |