Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultured human cells

Here, we present a chromatin-immunoprecipitation-based protocol to quantify the recruitment of proteins adjacent to site-specific DNA double-strand breaks (DSBs), such as proteins involved in DSB repair. We describe steps to induce DSBs in U2OS osteosarcoma cells stably expressing the restriction en...

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Detalles Bibliográficos
Autores principales: Sharma, Ajit K., Fitieh, Amira Mohammed, Locke, Andrew J., Ali, Jana Yasser Hafez, Ismail, Ismail Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758495/
https://www.ncbi.nlm.nih.gov/pubmed/36520630
http://dx.doi.org/10.1016/j.xpro.2022.101917

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758495/
https://www.ncbi.nlm.nih.gov/pubmed/36520630
http://dx.doi.org/10.1016/j.xpro.2022.101917

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