Quantification of protein enrichment at site-specific DNA double-strand breaks by chromatin immunoprecipitation in cultured human cells

Here, we present a chromatin-immunoprecipitation-based protocol to quantify the recruitment of proteins adjacent to site-specific DNA double-strand breaks (DSBs), such as proteins involved in DSB repair. We describe steps to induce DSBs in U2OS osteosarcoma cells stably expressing the restriction en...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharma, Ajit K., Fitieh, Amira Mohammed, Locke, Andrew J., Ali, Jana Yasser Hafez, Ismail, Ismail Hassan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9758495/
https://www.ncbi.nlm.nih.gov/pubmed/36520630
http://dx.doi.org/10.1016/j.xpro.2022.101917

Ejemplares similares

Ejemplares similares