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Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses
Experimental vaccines for the deadly zoonotic Nipah (NiV), Hendra (HeV), and Ebola (EBOV) viruses have focused on targeting individual viruses, although their geographical and bat reservoir host overlaps warrant creation of multivalent vaccines. Here we explored whether replication-incompetent pseud...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759047/ https://www.ncbi.nlm.nih.gov/pubmed/36528644 http://dx.doi.org/10.1038/s41541-022-00588-5 |
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author | Ithinji, Duncan G. Buchholz, David W. Ezzatpour, Shahrzad Monreal, I. Abrrey Cong, Yu Sahler, Julie Bangar, Amandip Singh Imbiakha, Brian Upadhye, Viraj Liang, Janie Ma, Andrew Bradel-Tretheway, Birgit Kaza, Benjamin Yeo, Yao Yu Choi, Eun Jin Johnston, Gunner P. Huzella, Louis Kollins, Erin Dixit, Saurabh Yu, Shuiqing Postnikova, Elena Ortega, Victoria August, Avery Holbrook, Michael R. Aguilar, Hector C. |
author_facet | Ithinji, Duncan G. Buchholz, David W. Ezzatpour, Shahrzad Monreal, I. Abrrey Cong, Yu Sahler, Julie Bangar, Amandip Singh Imbiakha, Brian Upadhye, Viraj Liang, Janie Ma, Andrew Bradel-Tretheway, Birgit Kaza, Benjamin Yeo, Yao Yu Choi, Eun Jin Johnston, Gunner P. Huzella, Louis Kollins, Erin Dixit, Saurabh Yu, Shuiqing Postnikova, Elena Ortega, Victoria August, Avery Holbrook, Michael R. Aguilar, Hector C. |
author_sort | Ithinji, Duncan G. |
collection | PubMed |
description | Experimental vaccines for the deadly zoonotic Nipah (NiV), Hendra (HeV), and Ebola (EBOV) viruses have focused on targeting individual viruses, although their geographical and bat reservoir host overlaps warrant creation of multivalent vaccines. Here we explored whether replication-incompetent pseudotyped vesicular stomatitis virus (VSV) virions or NiV-based virus-like particles (VLPs) were suitable multivalent vaccine platforms by co-incorporating multiple surface glycoproteins from NiV, HeV, and EBOV onto these virions. We then enhanced the vaccines’ thermotolerance using carbohydrates to enhance applicability in global regions that lack cold-chain infrastructure. Excitingly, in a Syrian hamster model of disease, the VSV multivalent vaccine elicited safe, strong, and protective neutralizing antibody responses against challenge with NiV, HeV, or EBOV. Our study provides proof-of-principle evidence that replication-incompetent multivalent viral particle vaccines are sufficient to provide protection against multiple zoonotic deadly viruses with high pandemic potential. |
format | Online Article Text |
id | pubmed-9759047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97590472022-12-19 Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses Ithinji, Duncan G. Buchholz, David W. Ezzatpour, Shahrzad Monreal, I. Abrrey Cong, Yu Sahler, Julie Bangar, Amandip Singh Imbiakha, Brian Upadhye, Viraj Liang, Janie Ma, Andrew Bradel-Tretheway, Birgit Kaza, Benjamin Yeo, Yao Yu Choi, Eun Jin Johnston, Gunner P. Huzella, Louis Kollins, Erin Dixit, Saurabh Yu, Shuiqing Postnikova, Elena Ortega, Victoria August, Avery Holbrook, Michael R. Aguilar, Hector C. NPJ Vaccines Article Experimental vaccines for the deadly zoonotic Nipah (NiV), Hendra (HeV), and Ebola (EBOV) viruses have focused on targeting individual viruses, although their geographical and bat reservoir host overlaps warrant creation of multivalent vaccines. Here we explored whether replication-incompetent pseudotyped vesicular stomatitis virus (VSV) virions or NiV-based virus-like particles (VLPs) were suitable multivalent vaccine platforms by co-incorporating multiple surface glycoproteins from NiV, HeV, and EBOV onto these virions. We then enhanced the vaccines’ thermotolerance using carbohydrates to enhance applicability in global regions that lack cold-chain infrastructure. Excitingly, in a Syrian hamster model of disease, the VSV multivalent vaccine elicited safe, strong, and protective neutralizing antibody responses against challenge with NiV, HeV, or EBOV. Our study provides proof-of-principle evidence that replication-incompetent multivalent viral particle vaccines are sufficient to provide protection against multiple zoonotic deadly viruses with high pandemic potential. Nature Publishing Group UK 2022-12-17 /pmc/articles/PMC9759047/ /pubmed/36528644 http://dx.doi.org/10.1038/s41541-022-00588-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Ithinji, Duncan G. Buchholz, David W. Ezzatpour, Shahrzad Monreal, I. Abrrey Cong, Yu Sahler, Julie Bangar, Amandip Singh Imbiakha, Brian Upadhye, Viraj Liang, Janie Ma, Andrew Bradel-Tretheway, Birgit Kaza, Benjamin Yeo, Yao Yu Choi, Eun Jin Johnston, Gunner P. Huzella, Louis Kollins, Erin Dixit, Saurabh Yu, Shuiqing Postnikova, Elena Ortega, Victoria August, Avery Holbrook, Michael R. Aguilar, Hector C. Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses |
title | Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses |
title_full | Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses |
title_fullStr | Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses |
title_full_unstemmed | Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses |
title_short | Multivalent viral particles elicit safe and efficient immunoprotection against Nipah Hendra and Ebola viruses |
title_sort | multivalent viral particles elicit safe and efficient immunoprotection against nipah hendra and ebola viruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759047/ https://www.ncbi.nlm.nih.gov/pubmed/36528644 http://dx.doi.org/10.1038/s41541-022-00588-5 |
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