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A simplified procedure for isolation of primary murine microglia

There are various approaches in which one can isolate microglia from murine brains, such as immunomagnetic, density gradient, FACS and differential adhesive methods. In this procedure a modified flask-tapping approach was used due to its simplicity and reproducibility. Our protocol requires only a s...

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Detalles Bibliográficos
Autores principales: Scott, Nathan, Witt, Ken, Schober, Joseph M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9761776/
https://www.ncbi.nlm.nih.gov/pubmed/36398847
http://dx.doi.org/10.2144/btn-2022-0054