Protocol for production and expression of chimeric bovine-human monoclonal antibodies

We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human...

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Detalles Bibliográficos
Autores principales: Heydarchi, Behnaz, Purcell, Damian F.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763940/
https://www.ncbi.nlm.nih.gov/pubmed/36595963
http://dx.doi.org/10.1016/j.xpro.2022.101911

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763940/
https://www.ncbi.nlm.nih.gov/pubmed/36595963
http://dx.doi.org/10.1016/j.xpro.2022.101911

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