Protocol for production and expression of chimeric bovine-human monoclonal antibodies

We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human...

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Detalles Bibliográficos
Autores principales: Heydarchi, Behnaz, Purcell, Damian F.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763940/
https://www.ncbi.nlm.nih.gov/pubmed/36595963
http://dx.doi.org/10.1016/j.xpro.2022.101911
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author Heydarchi, Behnaz
Purcell, Damian F.J.
author_facet Heydarchi, Behnaz
Purcell, Damian F.J.
author_sort Heydarchi, Behnaz
collection PubMed
description We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human cell lines. This protocol provides an efficient step-by-step methodology to produce HIV-1 chimeric mAbs and could be widely adapted for other antigens. For complete details on the use and execution of this protocol, please refer to Heydarchi et al. (2022).(1)
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spelling pubmed-97639402022-12-21 Protocol for production and expression of chimeric bovine-human monoclonal antibodies Heydarchi, Behnaz Purcell, Damian F.J. STAR Protoc Protocol We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human cell lines. This protocol provides an efficient step-by-step methodology to produce HIV-1 chimeric mAbs and could be widely adapted for other antigens. For complete details on the use and execution of this protocol, please refer to Heydarchi et al. (2022).(1) Elsevier 2022-12-09 /pmc/articles/PMC9763940/ /pubmed/36595963 http://dx.doi.org/10.1016/j.xpro.2022.101911 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Heydarchi, Behnaz
Purcell, Damian F.J.
Protocol for production and expression of chimeric bovine-human monoclonal antibodies
title Protocol for production and expression of chimeric bovine-human monoclonal antibodies
title_full Protocol for production and expression of chimeric bovine-human monoclonal antibodies
title_fullStr Protocol for production and expression of chimeric bovine-human monoclonal antibodies
title_full_unstemmed Protocol for production and expression of chimeric bovine-human monoclonal antibodies
title_short Protocol for production and expression of chimeric bovine-human monoclonal antibodies
title_sort protocol for production and expression of chimeric bovine-human monoclonal antibodies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9763940/
https://www.ncbi.nlm.nih.gov/pubmed/36595963
http://dx.doi.org/10.1016/j.xpro.2022.101911
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