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Ribosome Stalling of N-Linked Glycoproteins in Cell-Free Extracts

[Image: see text] Ribosome display is a powerful in vitro method for selection and directed evolution of proteins expressed from combinatorial libraries. However, the ability to display proteins with complex post-translational modifications such as glycosylation is limited. To address this gap, we d...

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Detalles Bibliográficos
Autores principales: Chung, Sean S., Bidstrup, Erik J., Hershewe, Jasmine M., Warfel, Katherine F., Jewett, Michael C., DeLisa, Matthew P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9764415/
https://www.ncbi.nlm.nih.gov/pubmed/36399685
http://dx.doi.org/10.1021/acssynbio.2c00311