Protocol to dissociate healthy and infected murine- and hamster-derived lung tissue for single-cell transcriptome analysis

In infectious disease research, single-cell RNA sequencing allows dissection of host-pathogen interactions. As a prerequisite, we provide a protocol to transform solid and complex organs such as lungs into representative diverse, viable single-cell suspensions. Our protocol describes performance of...

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Detalles Bibliográficos
Autores principales: Pennitz, Peter, Goekeri, Cengiz, Trimpert, Jakob, Wyler, Emanuel, Ebenig, Aileen, Weissfuss, Chantal, Mühlebach, Michael D., Witzenrath, Martin, Nouailles, Geraldine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765304/
https://www.ncbi.nlm.nih.gov/pubmed/36542521
http://dx.doi.org/10.1016/j.xpro.2022.101957

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765304/
https://www.ncbi.nlm.nih.gov/pubmed/36542521
http://dx.doi.org/10.1016/j.xpro.2022.101957

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