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Protocol to dissociate healthy and infected murine- and hamster-derived lung tissue for single-cell transcriptome analysis
In infectious disease research, single-cell RNA sequencing allows dissection of host-pathogen interactions. As a prerequisite, we provide a protocol to transform solid and complex organs such as lungs into representative diverse, viable single-cell suspensions. Our protocol describes performance of...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9765304/ https://www.ncbi.nlm.nih.gov/pubmed/36542521 http://dx.doi.org/10.1016/j.xpro.2022.101957 |