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STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity

Transcription is a dynamic process. To detect the dynamic relationship among protein clusters of RNA polymerase II and coactivators, gene loci, and transcriptional activity, we insert an MS2 repeat, a TetO repeat, and inteins with a selection marker just downstream of the transcription start site. B...

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Detalles Bibliográficos
Autores principales: Ohishi, Hiroaki, Shimada, Seiru, Uchino, Satoshi, Li, Jieru, Sato, Yuko, Shintani, Manabu, Owada, Hitoshi, Ohkawa, Yasuyuki, Pertsinidis, Alexandros, Yamamoto, Takashi, Kimura, Hiroshi, Ochiai, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768169/
https://www.ncbi.nlm.nih.gov/pubmed/36539402
http://dx.doi.org/10.1038/s41467-022-35286-2