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An optimized protocol for the isolation of rare stromal cell populations from the mouse spleen

Lymphoid tissue stromal cells are important regulators of spleen homeostasis and immune responses. Here, we present an optimized protocol that describes the digestion and enrichment steps for the isolation and analysis of rare populations of stromal cells, including fibroblastic reticular cells, per...

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Detalles Bibliográficos
Autores principales: Alexandre, Yannick O., Mueller, Scott N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768415/
https://www.ncbi.nlm.nih.gov/pubmed/36595952
http://dx.doi.org/10.1016/j.xpro.2022.101923