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An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia coli BW3KD strain with higher transformation...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769673/ https://www.ncbi.nlm.nih.gov/pubmed/36317996 http://dx.doi.org/10.1128/spectrum.02497-22 |