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An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency

DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia coli BW3KD strain with higher transformation...

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Detalles Bibliográficos
Autores principales:	Yang, Yuqing, Liu, Menghui, Wang, Tianqi, Wang, Qian, Liu, Huaiwei, Xun, Luying, Xia, Yongzhen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2022
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769673/ https://www.ncbi.nlm.nih.gov/pubmed/36317996 http://dx.doi.org/10.1128/spectrum.02497-22

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