High-Fidelity Cytosine Base Editing in a GC-Rich Corynebacterium glutamicum with Reduced DNA Off-Target Editing Effects

Genome editing technology is a powerful tool for programming microbial cell factories. However, rat APOBEC1-derived cytosine base editor (CBE) that converts C•G to T•A at target genes induced DNA off-targets, regardless of single-guide RNA (sgRNA) sequences. Although the high efficiencies of the bac...

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Detalles Bibliográficos
Autores principales: Heo, Yu Been, Hwang, Gue-Ho, Kang, Seok Won, Bae, Sangsu, Woo, Han Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769817/
https://www.ncbi.nlm.nih.gov/pubmed/36374037
http://dx.doi.org/10.1128/spectrum.03760-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9769817/
https://www.ncbi.nlm.nih.gov/pubmed/36374037
http://dx.doi.org/10.1128/spectrum.03760-22

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