Native holdup (nHU) to measure binding affinities from cell extracts

Characterizing macromolecular interactions is essential for understanding cellular processes, yet most methods currently used to detect protein interactions from cells are qualitative. Here, we introduce the native holdup (nHU) approach to estimate equilibrium binding constants of protein interactio...

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Detalles Bibliográficos
Autores principales: Zambo, Boglarka, Morlet, Bastien, Negroni, Luc, Trave, Gilles, Gogl, Gergo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Biomedicine and Life Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9770967/
https://www.ncbi.nlm.nih.gov/pubmed/36542723
http://dx.doi.org/10.1126/sciadv.ade3828

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9770967/
https://www.ncbi.nlm.nih.gov/pubmed/36542723
http://dx.doi.org/10.1126/sciadv.ade3828

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