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Efficient modification and preparation of circular DNA for expression in cell culture

DNA plasmids are an essential tool for delivery and expression of RNAs and proteins in cell culture experiments. The preparation of plasmids typically involves a laborious process of bacterial cloning, validation, and purification. While the expression plasmids can be designed and ordered from the c...

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Detalles Bibliográficos
Autores principales: Oliynyk, Roman Teo, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9772414/
https://www.ncbi.nlm.nih.gov/pubmed/36543890
http://dx.doi.org/10.1038/s42003-022-04363-z