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Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach

[Image: see text] Lipopeptide (LP) biosurfactants from microbes have the potential to gradually replace chemical synthetic surfactants and fit the contemporary green and sustainable industrial production concept. However, their active participation is comparatively low in the global market pertainin...

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Autores principales: Singh, Sanju, Sequeira, Rosy Alphons, Kumar, Pankaj, Ghadge, Vishal A., Vaghela, Pradipkumar, Mohanty, Ajeet Kumar, Ghosh, Arup, Prasad, Kamalesh, Shinde, Pramod B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774373/
https://www.ncbi.nlm.nih.gov/pubmed/36570225
http://dx.doi.org/10.1021/acsomega.2c05587
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author Singh, Sanju
Sequeira, Rosy Alphons
Kumar, Pankaj
Ghadge, Vishal A.
Vaghela, Pradipkumar
Mohanty, Ajeet Kumar
Ghosh, Arup
Prasad, Kamalesh
Shinde, Pramod B.
author_facet Singh, Sanju
Sequeira, Rosy Alphons
Kumar, Pankaj
Ghadge, Vishal A.
Vaghela, Pradipkumar
Mohanty, Ajeet Kumar
Ghosh, Arup
Prasad, Kamalesh
Shinde, Pramod B.
author_sort Singh, Sanju
collection PubMed
description [Image: see text] Lipopeptide (LP) biosurfactants from microbes have the potential to gradually replace chemical synthetic surfactants and fit the contemporary green and sustainable industrial production concept. However, their active participation is comparatively low in the global market pertaining to their low yield in microbial broth and costly downstream processes arising due to tedious isolation and purification methods. Herein, an efficient extraction method is developed that utilizes an aqueous biphasic system (ABS) comprising ionic liquids and polypropylene glycol 400 (PPG) to selectively extract a mixture of cyclic lipopeptides, namely, surfactin and fengycin from the culture broth of Bacillus amyloliquefaciens 5NPA-1, isolated from the halophyte Salicornia brachiata Roxb. Out of four different ABSs, the ABS composed of 2-hydroxyethyl ammonium formate and PPG displayed a maximum extraction efficiency of 82.30%. PPG-rich phase containing lipopeptides exhibited excellent antimicrobial and mosquito larvicidal properties with no toxic effect on plants. The developed method is simple, novel and accelerates the application of cyclic lipopeptides produced by the microbial source.
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spelling pubmed-97743732022-12-23 Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach Singh, Sanju Sequeira, Rosy Alphons Kumar, Pankaj Ghadge, Vishal A. Vaghela, Pradipkumar Mohanty, Ajeet Kumar Ghosh, Arup Prasad, Kamalesh Shinde, Pramod B. ACS Omega [Image: see text] Lipopeptide (LP) biosurfactants from microbes have the potential to gradually replace chemical synthetic surfactants and fit the contemporary green and sustainable industrial production concept. However, their active participation is comparatively low in the global market pertaining to their low yield in microbial broth and costly downstream processes arising due to tedious isolation and purification methods. Herein, an efficient extraction method is developed that utilizes an aqueous biphasic system (ABS) comprising ionic liquids and polypropylene glycol 400 (PPG) to selectively extract a mixture of cyclic lipopeptides, namely, surfactin and fengycin from the culture broth of Bacillus amyloliquefaciens 5NPA-1, isolated from the halophyte Salicornia brachiata Roxb. Out of four different ABSs, the ABS composed of 2-hydroxyethyl ammonium formate and PPG displayed a maximum extraction efficiency of 82.30%. PPG-rich phase containing lipopeptides exhibited excellent antimicrobial and mosquito larvicidal properties with no toxic effect on plants. The developed method is simple, novel and accelerates the application of cyclic lipopeptides produced by the microbial source. American Chemical Society 2022-12-09 /pmc/articles/PMC9774373/ /pubmed/36570225 http://dx.doi.org/10.1021/acsomega.2c05587 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Singh, Sanju
Sequeira, Rosy Alphons
Kumar, Pankaj
Ghadge, Vishal A.
Vaghela, Pradipkumar
Mohanty, Ajeet Kumar
Ghosh, Arup
Prasad, Kamalesh
Shinde, Pramod B.
Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach
title Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach
title_full Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach
title_fullStr Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach
title_full_unstemmed Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach
title_short Selective Partition of Lipopeptides from Fermentation Broth: A Green and Sustainable Approach
title_sort selective partition of lipopeptides from fermentation broth: a green and sustainable approach
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9774373/
https://www.ncbi.nlm.nih.gov/pubmed/36570225
http://dx.doi.org/10.1021/acsomega.2c05587
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