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Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours

An important research target is improving the health benefits of traditional Mediterranean, durum wheat-based foods using innovative raw materials. In this study, we characterised wholemeal flours obtained from a traditional durum wheat cv. Svevo, two innovative durum wheat varieties (Svevo-High Amy...

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Autores principales: Romano, Giuseppe, Del Coco, Laura, Milano, Francesco, Durante, Miriana, Palombieri, Samuela, Sestili, Francesco, Visioni, Andrea, Jilal, Abderrazek, Fanizzi, Francesco Paolo, Laddomada, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777840/
https://www.ncbi.nlm.nih.gov/pubmed/36553812
http://dx.doi.org/10.3390/foods11244070
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author Romano, Giuseppe
Del Coco, Laura
Milano, Francesco
Durante, Miriana
Palombieri, Samuela
Sestili, Francesco
Visioni, Andrea
Jilal, Abderrazek
Fanizzi, Francesco Paolo
Laddomada, Barbara
author_facet Romano, Giuseppe
Del Coco, Laura
Milano, Francesco
Durante, Miriana
Palombieri, Samuela
Sestili, Francesco
Visioni, Andrea
Jilal, Abderrazek
Fanizzi, Francesco Paolo
Laddomada, Barbara
author_sort Romano, Giuseppe
collection PubMed
description An important research target is improving the health benefits of traditional Mediterranean, durum wheat-based foods using innovative raw materials. In this study, we characterised wholemeal flours obtained from a traditional durum wheat cv. Svevo, two innovative durum wheat varieties (Svevo-High Amylose and Faridur), the naked barley cv. Chifaa and the elite lentil line 6002/ILWL118/1-1, evaluating them for targeted phytochemicals, untargeted metabolomics fingerprints and antioxidant capacity. To this aim, individual phenolic acids, flavonoids, tocochromanols and carotenoids were identified and quantified through HPLC-DAD, and the antioxidant capacities of both the extracts and whole meals were detected by ABTS assays. An untargeted metabolomics fingerprinting of the samples was conducted through NMR spectroscopy. Results showed that the innovative materials improved phytochemical profiles and antioxidant capacity compared to Svevo. In particular, Svevo-HA and Faridur had higher contents of ferulic and sinapic acids, β-tocotrienol and lutein. Moreover, Chifaa is a rich source of phenolic acids, β-tocopherols, lutein and zeaxanthin whereas lentil of flavonoids (i.e., catechin and procyanidin B2). The NMR profiles of Svevo-HA and Faridur showed a significant reduction of sugar content, malate and tryptophan compared to that of Svevo. Finally, substantial differences characterised the lentil profiles, especially for citrate, trigonelline and phenolic resonances of secondary metabolites, such as catechin-like compounds. Overall, these results support the potential of the above innovative materials to renew the health value of traditional Mediterranean durum wheat-based products.
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spelling pubmed-97778402022-12-23 Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours Romano, Giuseppe Del Coco, Laura Milano, Francesco Durante, Miriana Palombieri, Samuela Sestili, Francesco Visioni, Andrea Jilal, Abderrazek Fanizzi, Francesco Paolo Laddomada, Barbara Foods Article An important research target is improving the health benefits of traditional Mediterranean, durum wheat-based foods using innovative raw materials. In this study, we characterised wholemeal flours obtained from a traditional durum wheat cv. Svevo, two innovative durum wheat varieties (Svevo-High Amylose and Faridur), the naked barley cv. Chifaa and the elite lentil line 6002/ILWL118/1-1, evaluating them for targeted phytochemicals, untargeted metabolomics fingerprints and antioxidant capacity. To this aim, individual phenolic acids, flavonoids, tocochromanols and carotenoids were identified and quantified through HPLC-DAD, and the antioxidant capacities of both the extracts and whole meals were detected by ABTS assays. An untargeted metabolomics fingerprinting of the samples was conducted through NMR spectroscopy. Results showed that the innovative materials improved phytochemical profiles and antioxidant capacity compared to Svevo. In particular, Svevo-HA and Faridur had higher contents of ferulic and sinapic acids, β-tocotrienol and lutein. Moreover, Chifaa is a rich source of phenolic acids, β-tocopherols, lutein and zeaxanthin whereas lentil of flavonoids (i.e., catechin and procyanidin B2). The NMR profiles of Svevo-HA and Faridur showed a significant reduction of sugar content, malate and tryptophan compared to that of Svevo. Finally, substantial differences characterised the lentil profiles, especially for citrate, trigonelline and phenolic resonances of secondary metabolites, such as catechin-like compounds. Overall, these results support the potential of the above innovative materials to renew the health value of traditional Mediterranean durum wheat-based products. MDPI 2022-12-16 /pmc/articles/PMC9777840/ /pubmed/36553812 http://dx.doi.org/10.3390/foods11244070 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Romano, Giuseppe
Del Coco, Laura
Milano, Francesco
Durante, Miriana
Palombieri, Samuela
Sestili, Francesco
Visioni, Andrea
Jilal, Abderrazek
Fanizzi, Francesco Paolo
Laddomada, Barbara
Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours
title Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours
title_full Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours
title_fullStr Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours
title_full_unstemmed Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours
title_short Phytochemical Profiling and Untargeted Metabolite Fingerprinting of the MEDWHEALTH Wheat, Barley and Lentil Wholemeal Flours
title_sort phytochemical profiling and untargeted metabolite fingerprinting of the medwhealth wheat, barley and lentil wholemeal flours
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9777840/
https://www.ncbi.nlm.nih.gov/pubmed/36553812
http://dx.doi.org/10.3390/foods11244070
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