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Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts
Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785805/ https://www.ncbi.nlm.nih.gov/pubmed/36560241 http://dx.doi.org/10.3390/s22249874 |
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author | Gutiérrez-Ortiz, Anggy Lusanna Vida, Veronica Peterka, Matjaž Tušar, Jasmina Berti, Federico Navarini, Luciano Forzato, Cristina |
author_facet | Gutiérrez-Ortiz, Anggy Lusanna Vida, Veronica Peterka, Matjaž Tušar, Jasmina Berti, Federico Navarini, Luciano Forzato, Cristina |
author_sort | Gutiérrez-Ortiz, Anggy Lusanna |
collection | PubMed |
description | Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition present in the literature, none of them are based on optical fluorescence, which is very interesting given its great sensitivity. In the present manuscript, fluorescent polymeric imprinted nanoparticles were synthetized following the non-covalent approach using hydrogenated 5-O-caffeoylquinic acid (H-5-CQA) as the template. The capability of the polymer to bind 5-CQA was evaluated by HPLC and fluorescence. A real sample of coffee extract was also analyzed to verify the selectivity of the polymer. Polymer fMIP01, containing 4-vinylpyridine and a naphtalimide derivative as monomers, showed a good response to the fluorescence quenching in the range 39 μM–80 mM. In the real sample, fMIP01 was able to selectively bind 5-CQA, while caffeine was not recognized. To demonstrate this, there is a promising system that can be exploited in the design of an optical sensor for 5-CQA detection. Polymer fMIP01 was immobilized by physical entrapment on a functionalized glass surface, showing a quenching of fluorescence with an increase of the CGA concentration between 156 μM and 40 mM. |
format | Online Article Text |
id | pubmed-9785805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-97858052022-12-24 Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts Gutiérrez-Ortiz, Anggy Lusanna Vida, Veronica Peterka, Matjaž Tušar, Jasmina Berti, Federico Navarini, Luciano Forzato, Cristina Sensors (Basel) Article Green coffee beans are particularly rich in chlorogenic acids (CGAs), and their identification and quantification are usually performed by HPLC, coupled with mass spectrometry (LC-MS). Although there are a few examples of molecularly imprinted polymers (MIPs) for chlorogenic acid (5-CQA) recognition present in the literature, none of them are based on optical fluorescence, which is very interesting given its great sensitivity. In the present manuscript, fluorescent polymeric imprinted nanoparticles were synthetized following the non-covalent approach using hydrogenated 5-O-caffeoylquinic acid (H-5-CQA) as the template. The capability of the polymer to bind 5-CQA was evaluated by HPLC and fluorescence. A real sample of coffee extract was also analyzed to verify the selectivity of the polymer. Polymer fMIP01, containing 4-vinylpyridine and a naphtalimide derivative as monomers, showed a good response to the fluorescence quenching in the range 39 μM–80 mM. In the real sample, fMIP01 was able to selectively bind 5-CQA, while caffeine was not recognized. To demonstrate this, there is a promising system that can be exploited in the design of an optical sensor for 5-CQA detection. Polymer fMIP01 was immobilized by physical entrapment on a functionalized glass surface, showing a quenching of fluorescence with an increase of the CGA concentration between 156 μM and 40 mM. MDPI 2022-12-15 /pmc/articles/PMC9785805/ /pubmed/36560241 http://dx.doi.org/10.3390/s22249874 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gutiérrez-Ortiz, Anggy Lusanna Vida, Veronica Peterka, Matjaž Tušar, Jasmina Berti, Federico Navarini, Luciano Forzato, Cristina Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts |
title | Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts |
title_full | Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts |
title_fullStr | Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts |
title_full_unstemmed | Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts |
title_short | Fluorescent Imprinted Nanoparticles for Sensing of Chlorogenic Acid in Coffee Extracts |
title_sort | fluorescent imprinted nanoparticles for sensing of chlorogenic acid in coffee extracts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9785805/ https://www.ncbi.nlm.nih.gov/pubmed/36560241 http://dx.doi.org/10.3390/s22249874 |
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