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A high-efficiency PEG-Ca(2+)-mediated transient transformation system for broccoli protoplasts
Transient transformation of plant protoplasts is an important method for studying gene function, subcellular localization and plant morphological development. In this study, an efficient transient transformation system was established by optimizing the plasmid concentration, PEG4000 mass concentrati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9790990/ https://www.ncbi.nlm.nih.gov/pubmed/36578340 http://dx.doi.org/10.3389/fpls.2022.1081321 |