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Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo

Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosis by macrophages in vitro and in vivo. We describe the preparation and co-culture of macrophages and cancer cells, followed by in vitro phagocytosis assay using flow cytometry and confocal microscopy, respecti...

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Detalles Bibliográficos
Autores principales: Xu, Chuan, Wu, Hong, Liu, Yiqiang, Li, Feifei, Manne, Rajesh Kumar, Lin, Hui-Kuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791397/
https://www.ncbi.nlm.nih.gov/pubmed/36520628
http://dx.doi.org/10.1016/j.xpro.2022.101940