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Protocol for detecting macrophage-mediated cancer cell phagocytosis in vitro and in vivo
Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosis by macrophages in vitro and in vivo. We describe the preparation and co-culture of macrophages and cancer cells, followed by in vitro phagocytosis assay using flow cytometry and confocal microscopy, respecti...
Autores principales: | Xu, Chuan, Wu, Hong, Liu, Yiqiang, Li, Feifei, Manne, Rajesh Kumar, Lin, Hui-Kuan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791397/ https://www.ncbi.nlm.nih.gov/pubmed/36520628 http://dx.doi.org/10.1016/j.xpro.2022.101940 |
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