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Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses

Investigating the immune attack on β cells is critical to understanding autoimmune diabetes. Here, we present a protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas, followed by mass cytometric analyses. This protocol can be used to analyze subsets of innate and adap...

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Detalles Bibliográficos
Autores principales: Nargis, Titli, Piñeros, Annie R., May, Sarah C., Tersey, Sarah A., Mirmira, Raghavendra G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791398/
https://www.ncbi.nlm.nih.gov/pubmed/36520629
http://dx.doi.org/10.1016/j.xpro.2022.101938