Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses

Investigating the immune attack on β cells is critical to understanding autoimmune diabetes. Here, we present a protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas, followed by mass cytometric analyses. This protocol can be used to analyze subsets of innate and adap...

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Detalles Bibliográficos
Autores principales: Nargis, Titli, Piñeros, Annie R., May, Sarah C., Tersey, Sarah A., Mirmira, Raghavendra G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791398/
https://www.ncbi.nlm.nih.gov/pubmed/36520629
http://dx.doi.org/10.1016/j.xpro.2022.101938
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author Nargis, Titli
Piñeros, Annie R.
May, Sarah C.
Tersey, Sarah A.
Mirmira, Raghavendra G.
author_facet Nargis, Titli
Piñeros, Annie R.
May, Sarah C.
Tersey, Sarah A.
Mirmira, Raghavendra G.
author_sort Nargis, Titli
collection PubMed
description Investigating the immune attack on β cells is critical to understanding autoimmune diabetes. Here, we present a protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas, followed by mass cytometric analyses. This protocol can be used to analyze subsets of innate and adaptive immune cells that play critical roles in autoimmune diabetes, with as few as 5 × 10(5) cells. This protocol can also be adapted to study resident immune cells from other tissues. For complete details on the use and execution of this protocol, please refer to Piñeros et al. (2022).(1)
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spelling pubmed-97913982022-12-27 Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses Nargis, Titli Piñeros, Annie R. May, Sarah C. Tersey, Sarah A. Mirmira, Raghavendra G. STAR Protoc Protocol Investigating the immune attack on β cells is critical to understanding autoimmune diabetes. Here, we present a protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas, followed by mass cytometric analyses. This protocol can be used to analyze subsets of innate and adaptive immune cells that play critical roles in autoimmune diabetes, with as few as 5 × 10(5) cells. This protocol can also be adapted to study resident immune cells from other tissues. For complete details on the use and execution of this protocol, please refer to Piñeros et al. (2022).(1) Elsevier 2022-12-14 /pmc/articles/PMC9791398/ /pubmed/36520629 http://dx.doi.org/10.1016/j.xpro.2022.101938 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Nargis, Titli
Piñeros, Annie R.
May, Sarah C.
Tersey, Sarah A.
Mirmira, Raghavendra G.
Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
title Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
title_full Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
title_fullStr Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
title_full_unstemmed Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
title_short Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
title_sort protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791398/
https://www.ncbi.nlm.nih.gov/pubmed/36520629
http://dx.doi.org/10.1016/j.xpro.2022.101938
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