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Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains

Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modif...

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Detalles Bibliográficos
Autores principales: Lee, Dongjin R., Zhang, Yajun, Rhodes, Christopher T., Petros, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791422/
https://www.ncbi.nlm.nih.gov/pubmed/36520627
http://dx.doi.org/10.1016/j.xpro.2022.101944