Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains

Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modif...

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Detalles Bibliográficos
Autores principales: Lee, Dongjin R., Zhang, Yajun, Rhodes, Christopher T., Petros, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791422/
https://www.ncbi.nlm.nih.gov/pubmed/36520627
http://dx.doi.org/10.1016/j.xpro.2022.101944
Descripción
Sumario:Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modified for any brain region and/or neural cell type. For complete details on the use and execution of this protocol, please refer to Lee et al. (2022),(1) Rhodes et al. (2022),(2) Mahadevan et al. (2021),(3) Ekins et al. (2020),(4) and Wester et al. (2019).(5)

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