Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains

Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modif...

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Detalles Bibliográficos
Autores principales: Lee, Dongjin R., Zhang, Yajun, Rhodes, Christopher T., Petros, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791422/
https://www.ncbi.nlm.nih.gov/pubmed/36520627
http://dx.doi.org/10.1016/j.xpro.2022.101944
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author Lee, Dongjin R.
Zhang, Yajun
Rhodes, Christopher T.
Petros, Timothy J.
author_facet Lee, Dongjin R.
Zhang, Yajun
Rhodes, Christopher T.
Petros, Timothy J.
author_sort Lee, Dongjin R.
collection PubMed
description Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modified for any brain region and/or neural cell type. For complete details on the use and execution of this protocol, please refer to Lee et al. (2022),(1) Rhodes et al. (2022),(2) Mahadevan et al. (2021),(3) Ekins et al. (2020),(4) and Wester et al. (2019).(5)
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spelling pubmed-97914222022-12-27 Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains Lee, Dongjin R. Zhang, Yajun Rhodes, Christopher T. Petros, Timothy J. STAR Protoc Protocol Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modified for any brain region and/or neural cell type. For complete details on the use and execution of this protocol, please refer to Lee et al. (2022),(1) Rhodes et al. (2022),(2) Mahadevan et al. (2021),(3) Ekins et al. (2020),(4) and Wester et al. (2019).(5) Elsevier 2022-12-14 /pmc/articles/PMC9791422/ /pubmed/36520627 http://dx.doi.org/10.1016/j.xpro.2022.101944 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Lee, Dongjin R.
Zhang, Yajun
Rhodes, Christopher T.
Petros, Timothy J.
Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
title Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
title_full Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
title_fullStr Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
title_full_unstemmed Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
title_short Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
title_sort generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791422/
https://www.ncbi.nlm.nih.gov/pubmed/36520627
http://dx.doi.org/10.1016/j.xpro.2022.101944
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