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Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains
Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modif...
Autores principales: | Lee, Dongjin R., Zhang, Yajun, Rhodes, Christopher T., Petros, Timothy J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9791422/ https://www.ncbi.nlm.nih.gov/pubmed/36520627 http://dx.doi.org/10.1016/j.xpro.2022.101944 |
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